thr77 Secrets
thr77 Secrets
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical position in endorsing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been described. Two more splice variants had been reported but in depth nucleotide sequence information and facts will not be but offered.
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Blocking peptides are peptides that bind specifically to your target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody.
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This guidebook outlines even more feasible optimization of cellular and immunoassay parameters to guarantee the absolute best results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Target Info Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation styles which lead into the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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